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Shrimple is based on the principle of a sandwich immunoassay.
The test device consists of a membrane strip and plastic case. A colored
monoclonal anti-WSSV antibody colloid gold conjugate (antibody - colloid
gold conjugate) pad is placed next to the sample pad region on the membrane
strip. The membrane strip is also pre-coated with different monoclonal
anti-WSSV antibody on the test (T) zone and anti-ImmnoglobulinG (IgG)
antibody on the control (C) zone.
In the absence of WSSV in the shrimp, the antibody-colloid gold conjugate moves
with the sample by capillary action along the membrane through the T zone until
it reaches the C zone. At this point, antibody-colloid gold conjugate reacts
with the pre-coated anti-IgG antibody to produce a visible pink colored band.
When only one pink colored band appears on the C zone, it means negative.
When the WSSV is present in the shrimp, the antibody-colloid gold conjugate reacts
with WSSV to produce conjugate-WSSV complex and moves until it reaches the T
zone. At this point, the complex reacts with the pre-coated different monoclonal
anti-WSSV antibody to produce a visible pink colored band. An excess free
antibody-colloid gold conjugate passes through the T zone until the C zone. At
this point, the excess antibody-colloid gold conjugate reacts with the pre-coated
anti-IgG antibody to produce a visible pink colored band. When the pink colored
band appears in both T zone and C zone, it means positive. |
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