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Outbreaks of white spot syndrome (WSS), causing serious mortality to cultured shrimp, have been occurring in the East Asia since 1992. WSS has spread widely, and caused serious damage to shrimp farms in the world. Detection of white spot syndrome virus (WSSV) in shrimps is very important for management of shrimp farms.
EnBio Shrimp Virus Detection kit, named Shrimple, is a lateral flow, one step, and immunoassay for WSSV detection in shrimps. Shrimple is fast and easy. The results are read visually without any instruments. The test system employs unique monoclonal antibodies to selectively identify WSSV in shrimps with a high degree of sensitivity. |
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Cost effective management tool for shrimp culture pond |
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Monoclonal antibody based detection |
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All in one. No additional special equipment is required |
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Results within 20 minutes |
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Room temperature preservation is possible |
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| 1.Sampling. |
Wash the shrimps that look sick or unhealthy with
clean water and prepare the testing sample.
And put them into a micro tube. When cutting off the
swimming legs is done by hands, wash your hands well
beforehand. |
2.Crush the
sample.
The number of testing
sample required of one microtube is as follows; |
Size of shrimp |
Testing sample |
Required number
of shrimps |
PL7 |
Whole body |
20 |
PL10 or less |
Whole body |
10 |
PL20 or less |
Whole body |
5 |
0.05g or less |
Whole body |
2 |
0.1g or less |
Whole body |
1 |
0.5g or less |
Whole body without head and carapace |
1 |
2g or less |
All legs |
1 |
3g or less |
All swimming legs |
1 |
5g or less |
Five swimming legs |
1 |
10g or less |
Three swimming legs |
1 |
20g or less |
Two swimming legs |
1 |
Over 20g |
One swimming leg |
1 |
* Adjust sample volume to about 0.1g each 1 test |
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| 3.Put 4 drops on round hole of Shrimple |
| Then settle them for five minutes and take the supernatant with the dropper and make four droppings into the round hole of Shrimple device. |
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| 4.Check the red band |
| The appearance of a red band at the C line of the Shrimple device means it is working properly. When the red band appears at the T line within 15minutes after the dropping,you may assume it means a positive result(WSSV infection). Not appearing means a negative result(no WSSV infection). |
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In Thailand WSSV Detection
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